LOCUS       40924_4744        5747 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pARG1U DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pARG1U
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5747)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5747)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5747)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             241..894
                     /pseudo
                     /gene="arg1"
                     /label=arg1
                     /note="NotI site is created at the middle of this fragment 
                     (551-558)."
     gene            241..894
                     /pseudo
                     /gene="arg1"
                     /label=arg1
     regulatory      899..1421
                     /gene="ura4"
                     /regulatory_class="promoter"
     CDS             1422..2213
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      2227..2594
                     /gene="ura4"
                     /regulatory_class="terminator"
     primer_bind     complement(2607..2623)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2836..3291
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3573..3677
                     /label=AmpR promoter
     CDS             3678..4535
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4709..5297
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5585..5606
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5621..5651
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5659..5675
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5683..5699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtcgtagct gctaaaggtg agggctccta tttgtttgac aaggaaggtc gtaaatacat
      301 tgacttcacc tctggcgtag ctgttacatc tttgggtcat gctcatcctg aagttgctag
      361 acttgccgct gatcaatgct ccaaattagt ccatagtagc aaccttttct acaacgagcc
      421 tgccattgag ctatctaatg tcattaataa ttctttggca aaaaatagcg gtattgctgg
      481 ccccacaaaa attttctttg ccaattgtgg tactgaggcc aatgagacag ccttgaagtt
      541 cgctcgtaag gcggccgcgt ttgagaagta tggagaaggg aagagtcaaa ttgtttactt
      601 taataactct ttccacggcc gttctttggg atctcttagt atcactgcga accccaaata
      661 taaacgtggt ttccaaccat tacttcctga tgtcgtccaa gccgtctata atgaccctgc
      721 atccatagag caatttgtca atgacaaaac tgcggctgtt attgtcgagc ccgttcaagg
      781 tgaaggtggt atttgtcctg ccaagcctga atttttgatt gctttgcgca aggcctgtga
      841 taaggttggt gcttctttaa tctatgatga aattcaatgc ggacttggtc gcagatctac
      901 aaatcccact ggctatatgt atgcatttgt gttaaaaaag tttgtataga ttatttaatc
      961 tactcagcat tctttctcta aataggaatt tgttacttaa tggagaaaaa aatgtttcga
     1021 tttacctagt gtatttgttt gtatactcac gtttaatttc aaacatccat tctatcttgt
     1081 gtaatttttg gcatggtgaa aaagataatc agccttataa tctttacaaa agtaagaaat
     1141 tctgtaaata agccttaatg cccttgcttt aaattaaaat ggttcttttt catgataatg
     1201 tttgcacttt gtgaatatat tttagatagt tctgtgaggt ataattaaga tgttttagag
     1261 acttatacaa ttttgtcttt ataaattctt aattgatttt accatcccag tttaactatg
     1321 cttcgtcggc atctctgcac atgtcgtgtt ttcttaccgt attgtcctac caagaacctc
     1381 ttttttgctt ggatcgaaat taaaggttta aaagcaaagt tatggatgct agagtatttc
     1441 aaagctattc agctagagct gaggggatga aaaatcccat tgccaaggaa ttgttggctt
     1501 tgatggaaga aaagcaaagc aacttgtcag tcgcggtcga tttgacgaag aaatccgaaa
     1561 tcttagaatt ggtagataaa attggaccct atgtctgtgt tatcaagaca catattgacg
     1621 ttgtcgagga tttcgaccag gatatggtag aaaaactggt ggccttaggt aaaaagcatc
     1681 gttttcttat ctttgaggat cgcaaattcg cagacattgg aaataccgtc aagctacaat
     1741 atgcatctgg tgtgtacaaa attgcttctt gggctcatat cacaaattgc catacagtgc
     1801 caggcgaggg tattatacaa ggcctcaaag aagttggttt acctttggga cgtggtctct
     1861 tgcttttggc tgaaatgtct tccaaaggct ctttggctac tggttcctac acagagaaaa
     1921 ccttagaatg gtttgagaag cataccgatt tttgctttgg ctttatagct ggtcgtcgat
     1981 ttcctaacct tcaaagcgac tacataacta tgtcccctgg tatcggcttg gatgttaaag
     2041 gagacgggct gggacagcaa tatcgtactc ctgaagaagt gattgtaaac tgcggtagcg
     2101 atatcatcat tgttggtcgt ggagtctatg gagctggtcg taatcctgtt gtcgaagcca
     2161 agagatatag agaagctggt tggaaggcat atcagcaaag actttctcag cattaaaaaa
     2221 agactaatgt aaaatttttt tggttggtta ttgaaaaagt cgatgccttg tttgcgtttg
     2281 ttttcctagg cgttttatgt cagaaggcat ttagaattag tatacaagta ctctttggta
     2341 aaattttatg tagcgactaa aatattaact attatagata aacaccttgg gaataaaaag
     2401 taatttgcta tagtaattta ttaaacatgc tcctacaaca ttaccacaat cttttctctt
     2461 ggattgacat tgaataagaa aagagtgaat ttttttagac ttgtaatgat aactatgtac
     2521 aaagccaatg aaagatgtat gtagatgaat gtaaaatacc atgtagacaa acaagataaa
     2581 aatcacgttt aaacgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa
     2641 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2701 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2761 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     2821 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2881 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2941 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3001 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     3061 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3121 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     3181 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3241 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     3301 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     3361 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     3421 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     3481 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3541 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3601 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3661 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3721 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3781 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3841 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3901 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     3961 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4021 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4081 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4141 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4201 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4261 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4321 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4381 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4441 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4501 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4561 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4621 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4681 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4741 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4801 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     4861 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4921 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4981 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5041 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5101 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5161 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5221 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5281 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5341 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5401 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5461 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5521 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5581 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     5641 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     5701 atgattacgc caagcttgca tgcctgcagg tcgactctag aggatcc
//
